Biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering

Hardy, John George and Torres-Rendon, Jose Guillermo and Leal-Egana, Aldo and Walther, Andreas and Schlaad, Helmut and Cölfen, Helmut and Scheibel, Thomas Rainer (2016) Biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering. Materials, 9 (7). ISSN 1996-1944

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Abstract

Materials based on biodegradable polyesters such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT) have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein is reported the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.

Item Type:
Journal Article
Journal or Publication Title:
Materials
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2500
Subjects:
ID Code:
80178
Deposited By:
Deposited On:
24 Jun 2016 08:20
Refereed?:
Yes
Published?:
Published
Last Modified:
02 Apr 2020 03:58