Holtman, Wessel L. and Roberts, Michael R. and Oppedijk, Berry J. and Testerink, Christa and Van Zeijl, Mieke J. and Wang, Mei (2000) 14-3-3 proteins intact with a 13-lipoxygenase, but not with a 9-lipoxygenase. FEBS Letters, 474 (1). pp. 48-52. ISSN 0014-5793
Full text not available from this repository.Abstract
Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.