Crystallization and diffraction of an Lhx4–Isl2 complex.

Gadd, Morgan Stuart and Langley, David B and Guss, J Mitchell and Matthews, Jacqueline M (2009) Crystallization and diffraction of an Lhx4–Isl2 complex. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 65 (2). pp. 151-153. ISSN 1744-3091

Abstract

A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Lhx4 tethered to a peptide region of Isl2 has been engineered, purified and crystallized. The monoclinic crystals belonged to space group P21, with unit-cell parameters a = 46.8, b = 88.7, c = 49.9 Å, β = 111.9°, and diffracted to 2.16 Å resolution.