Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Lišková, Jana and Douglas, Timothy E.L. and Wijnants, Robbe and Samal, Sangram Keshari and Mendes, Ana C. and Chronakis, Ioannis and Bačáková, Lucie and Skirtach, Andre G. (2018) Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. Materials Letters, 214. pp. 186-189. ISSN 0167-577X

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Abstract

Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

Item Type:
Journal Article
Journal or Publication Title:
Materials Letters
Additional Information:
This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 214, 2018 DOI: 10.1016/j.matlet.2017.12.004
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100/3104
Subjects:
ID Code:
89125
Deposited By:
Deposited On:
05 Jan 2018 13:46
Refereed?:
Yes
Published?:
Published
Last Modified:
30 Oct 2020 04:54