Lišková, Jana and Douglas, Timothy E.L. and Wijnants, Robbe and Samal, Sangram Keshari and Mendes, Ana C. and Chronakis, Ioannis and Bačáková, Lucie and Skirtach, Andre G. (2018) Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. Materials Letters, 214. pp. 186-189. ISSN 0167-577X
MLBLUE_D_17_01720R1_1_.pdf - Accepted Version
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Abstract
Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.