Gholipourmalekabadi, Mazaher and Samadikuchaksaraei, Ali and Seifalian, Alexander and Urbanska, Aleksandra and Ghanbarian, Hossein and Hardy, John George and Omrani, Mir and Mozafari, Masoud and Reis, Rui and Kundu, Subhas (2018) Silk fibroin/amniotic membrane 3D bi-layered artificial skin. Biomedical Materials, 13 (3): 035003. ISSN 1748-6041
Gholipourmalekabadi_et_al_2017_Biomed._Mater._10.1088_1748_605X_aa999b.pdf - Accepted Version
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Abstract
Burn injury has been reported to be an important cause of morbidity and mortality and it is still considered as unmet clinical need. Although there is a myriad of effective stem cells suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a 3D bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bilayer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate a significant improved mechanical properties of the AM/ESF compared to the AM alone. Both AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against the Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors VEGFa and bFGF when cultured on the AM/ESF for 7 days in comparison with AM alone. The results suggest that AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in clinical setting in skin regeneration.