Effects of 4-Nonylphenol on spermatogenesis and induction of testicular apoptosis through oxidative stress-related pathways

Duan, Peng and Hu, Chunhui and Butler, Holly and Chen, Wei and Huang, Wenting and Tang, Sha and Zhou, Wei and Yuan, Meng and Shi, Yuqin and Martin, Francis Luke and Yang, Kedi (2016) Effects of 4-Nonylphenol on spermatogenesis and induction of testicular apoptosis through oxidative stress-related pathways. Reproductive Toxicology, 62. pp. 27-38. ISSN 0890-6238

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This study tested the hypothesis that prepubertal exposure to 4-Nonylphenol (NP) affects reproductive function in male rats. Twenty-four rats at five-weeks-old were randomly divided into four groups and treated with NP at varying concentrations (0, 5, 20, and 60 mg/kg/2d) for thirty days by intra-peritoneal injection. 60 mg/kg NP induced spermatogenic degeneration and pronounced deficits in epididymal sperm count, motility and function, whereas potentially stimulatory effects were observed at 5 NP mg/kg. Moreover, 60 mg/kg NP resulted in a significant reduction in fructose, FSH and LH; induced apoptosis related to oxidative stress; inhibited mRNA and protein levels of Bcl-2 and PCNA; as well as the additional up-regulation of p53, Bax, Apaf-1, cytochrome c, cleaved-caspase-3, Fas and FasL expression. Our data suggest potentially hormetic effects of NP on spermatogenic function. High-dose NP impairs testicular development and function by reducing cell proliferation and inducing apoptosis involving oxidative stress-related p53-Bcl-2/Bax and −Fas/FasL pathways.

Item Type:
Journal Article
Journal or Publication Title:
Reproductive Toxicology
Additional Information:
This is the author’s version of a work that was accepted for publication in Reproductive Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Reproductive Toxicology, 62 2016 DOI: 10.1016/j.reprotox.2016.04.016
Uncontrolled Keywords:
?? 4-nonylphenolhormetic effectreproductive toxicityoxidative stressapoptosisintrinsic and extrinsic apoptotic pathwaysratstoxicology ??
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Deposited On:
27 Apr 2016 15:58
Last Modified:
07 Apr 2024 23:38