Genetic manipulation of Rubisco:chromatium vinosum rbcL is expressed in Nicotiana tabacum but does not form a functional protein

Madgwick, Pippa J. and Colliver, S. P. and Banks, F. M. and Habash, D. Z. and Dulieu, H. and Parry, M. A J and Paul, M. J. (2002) Genetic manipulation of Rubisco:chromatium vinosum rbcL is expressed in Nicotiana tabacum but does not form a functional protein. Annals of Applied Biology, 140 (1). pp. 13-19. ISSN 0003-4746

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Abstract

N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbcL and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits were detectable in any line.

Item Type:
Journal Article
Journal or Publication Title:
Annals of Applied Biology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1100/1102
Subjects:
ID Code:
76022
Deposited By:
Deposited On:
21 Oct 2015 05:03
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 09:25