Genetic manipulation of Rubisco : chromatium vinosum rbcL is expressed in Nicotiana tabacum but does not form a functional protein

Madgwick, Pippa J. and Colliver, S. P. and Banks, F. M. and Habash, D. Z. and Dulieu, H. and Parry, M. A J and Paul, M. J. (2002) Genetic manipulation of Rubisco : chromatium vinosum rbcL is expressed in Nicotiana tabacum but does not form a functional protein. Annals of Applied Biology, 140 (1). pp. 13-19. ISSN 0003-4746

Abstract

N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbcL and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits were detectable in any line.