Intercalation of benzoxaborolate anions in layered double hydroxides : toward hybrid formulations for benzoxaborole drugs

Sene, Saad and Bégu, Sylvie and Gervais, Christel and Renaudin, Guillaume and Mesbah, Adel and Smith, Mark E. and Mutin, P. Hubert and Van Der Lee, Arie and Nedelec, Jean-marie and Bonhomme, Christian and Laurencin, Danielle (2015) Intercalation of benzoxaborolate anions in layered double hydroxides : toward hybrid formulations for benzoxaborole drugs. Chemistry of Materials, 27 (4). pp. 1242-1254. ISSN 0897-4756

Abstract

Benzoxaboroles are a family of cyclic derivatives of boronic acids, whose reactivity makes them interesting candidates for the development of novel drugs. In this study, we describe the preparation of the first hybrid organic–inorganic materials involving benzoxaboroles, as a first step toward their use as new formulations for such drugs. The materials were prepared by intercalation of the simplest benzoxaborole (C7H6BO(OH), BBzx) or of its fluorinated analogue (C7H5FBO(OH), AN2690, a recently developed antifungal drug), both taken under their anionic form (benzoxaborolate), into a biodegradable inorganic matrix (a Mg–Al layered double hydroxide). Extensive characterization was carried out on the materials, notably by powder X-ray diffraction and multinuclear (11B, 27Al, 13C, 19F, 25Mg, and 1H) solid state NMR, in order to describe their structure, particularly in the vicinity of the organoboron species. Three crystalline phases involving benzoxaborolate anions in association with Ca2+ or Mg2+ cations were also prepared as part of this work (Mg(C7H6BO(OH)2)2·10H2O, Mg(C7H6BO(OH)2)2·7H2O and Ca3(C7H6BO(OH)2)5(C7H6BO2)·3H2O), in order to assist in the interpretation of the spectroscopic data. A DFT computational model of the interlayer space was proposed, which is consistent with the experimental observations. Several properties of the materials were then determined with a view of using them as part of novel formulations, namely the maximum loading capacity toward benzoxaborol(at)es, the optimal storage conditions, and the release kinetics in simulated physiological media. All in all, this study serves as a benchmark not only for the development of novel formulations for benzoxaborole drugs, but more generally for the preparation of a novel class of organic–inorganic materials involving benzoxaborol(at)es.