Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens

Cunningham, Charles and Gatherer, Derek and Hilfrich, Birgitta and Baluchova, Katarina and Dargan, Derrick J. and Thomson, Marian and Griffiths, Paul D. and Wilkinson, Gavin W. G. and Schulz, Thomas F. and Davison, Andrew J. (2010) Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens. Journal of General Virology, 91 (3). pp. 605-615. ISSN 0022-1317

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Abstract

We have assessed two approaches to sequencing complete human cytomegalovirus (HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157, HAN13, HAN20 and HAN38) or clinical specimens (strains JP and 3301). The first approach involved amplifying genomes from the DNA samples as overlapping PCR products, sequencing these by the Sanger method, acquiring reads from a capillary instrument and assembling these using the Staden programs. The second approach involved generating sequence data from the DNA samples by using an Illumina Genome Analyzer (IGA), processing the filtered reads by reference-independent (de novo) assembly, utilizing the resulting sequence to direct reference-dependent assembly of the same data and finishing by limited PCR sequencing. Both approaches were successful. In particular, the investigation demonstrated the utility of IGA data for efficiently sequencing genomes from clinical samples containing as little as 3 % HCMV DNA. Analysis of the genome sequences obtained showed that each of the strains grown in cell culture was a mutant. Certain of the mutations were shared among strains from independent clinical sources, thus suggesting that they may have arisen in a common ancestor during natural infection. Moreover, one of the strains (JP) sequenced directly from a clinical specimen was mutated in two genes, one of which encodes a proposed immune-evasion function, viral interleukin-10. These observations imply that HCMV mutants exist in human infections.

Item Type:
Journal Article
Journal or Publication Title:
Journal of General Virology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2406
Subjects:
?? VIROLOGY ??
ID Code:
65178
Deposited By:
Deposited On:
17 Jun 2013 08:52
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Sep 2023 01:06