Exploring the effects of a multicomponent phytoceutical health product in a Drosophila melanogaster ageing and repetitive concussion model

Alanazi, Norah and Fraysse, Audrey and English, Elisabeth A and Quigley, Sophia and Felix, Juri A and Howes, Melanie-Jayne R and Glassbrook, Dan and Hind, Karen and Chazot, Paul (2025) Exploring the effects of a multicomponent phytoceutical health product in a Drosophila melanogaster ageing and repetitive concussion model. Journal of Pharmacy and Pharmacology. ISSN 0022-3573

Abstract

Objectives: This study aims to investigate the long-term effects of multiple concussions on physical function and oxidative stress, as well as the potential protective effects of a novel phytoceutical product (CONKA1) on ageing and brain health of Drosophila melanogaster. Methods: Adult female D. melanogaster flies were exposed to repeated concussive events using a high-impact trauma (HIT) device. The effects of these impacts were assessed on lifespan, motor activity, and levels of reactive oxygen and nitrogen species (RONS) in both the brain and body. The flies were administered a multicomponent supplement (CONKA1), comprising Withania somnifera, Curcuma longa, Melissa officinalis, Rhodiola rosea, and Vaccinium myrtillus, and outcomes were compared to untreated controls. Key findings: CONKA1 improved the lifespan and motor activity and reduced oxidative stress in ageing flies. Repetitive concussion hits significantly increased long-term mobility deficits, shortened lifespan, and exacerbated oxidative stress in both the brain and body, above and beyond normal ageing. CONKA1 demonstrated mitigation of extra motor deficits, reduced lifespan, and exacerbated oxidative stress in both the brain and body of the flies. Conclusions: The findings validate that D. melanogaster is a suitable system to mimic the effects of repetitive concussions and evaluate the effects of novel health products.