Enhanced abundance of generalist and litter saprotrophs explain increased tropical forest soil carbon with long-term nitrogen deposition

Lu, Z. and Li, H. and Sayer, E.J. and Liu, Z. and Li, L. and Chen, Y. and Qin, G. and Li, J. and Zhou, J. and Huang, X. and Zhang, J. and Wu, J. and Thapa, P. and Wang, F. (2023) Enhanced abundance of generalist and litter saprotrophs explain increased tropical forest soil carbon with long-term nitrogen deposition. Functional Ecology, 37 (8). pp. 2282-2296. ISSN 0269-8463

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Abstract

Nitrogen (N) deposition is a pervasive anthropogenic change that can alter the dynamics and storage of carbon (C) in tropical soils by altering the transformation of plant litter C to soil organic C (SOC). The response of fungi may be particularly important for explaining how N deposition affects SOC storage in tropical forests because they are the primary decomposers of plant litter. Fungi can be grouped into different ecological guilds, or functional groups, which often have contrasting responses to the same environmental change. However, little is known if shifts in fungal guilds and their functions explain increased tropical forest SOC storage under N deposition. We addressed this knowledge gap by assessing the effects of long‐term (6 years) experimental N addition (+N) on fungal guilds, their functions and SOC fractions in a tropical forest. Total SOC, dissolved organic C (DOC), particulate organic C and fungal necromass C were all higher in +N soils. The fungal community in +N soils was dominated by generalist saprotrophs, and leaf saprotroph abundance increased nearly 93‐fold compared to controls. These changes were accompanied by an increase in lignocellulolytic enzymes, indicating accelerated decomposition of recalcitrant litter C compounds. Chitosanase, which catalyses synthesis of glucosamine, was 1.37× higher in +N soils than controls; correspondingly, fungal necromass C contributed 1.42× more to SOC. Greater abundance of saprotrophic enzymes involved in organic acid synthesis in +N soils was associated with higher concentration of Ca2+ and DOC. We propose that N deposition increases SOC storage by enhancing saprotroph abundance and activity, whereby increased organic acid production supports fungal growth both directly and indirectly via the release of nutrients and DOC, resulting in greater inputs of necromass to the soil. Our findings highlight the importance of considering shifts in guild‐level fungal abundance and function to investigate changes in SOC dynamics and storage. Read the free Plain Language Summary for this article on the Journal blog.