Dopamine/mucin-1 functionalized electro-active carbon nanotubes as a probe for direct competitive electrochemical immunosensing of breast cancer biomarker

Rashid, S. and Nawaz, M.H. and Rehman, I.U. and Hayat, A. and Marty, J.L. (2021) Dopamine/mucin-1 functionalized electro-active carbon nanotubes as a probe for direct competitive electrochemical immunosensing of breast cancer biomarker. Sensors and Actuators B: Chemical, 330. ISSN 0925-4005

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Abstract

Mucin-1 (MUC-1) is associated with a broad range of human epithelia including gastric, lung and colorectal. In this work, a direct competitive electrochemical immunosensor based on gelatin modified transduction platform was designed. Dopamine (DA)/mucin-1 functionalized electro-active carbon nanotubes were employed as signal generating probes in the construction of electrochemical immunosensor for early stage diagnosis of breast cancer. The gelatin modified electrode served as a support to immobilize antibody (anti-MUC-1), while electrochemical response of functionalized electro-active carbon nano probes was used for quantitative measurement of MUC-1. Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were carried out to characterize the transduction surface at different fabrication steps. The developed immunosensor permitted the detection of MUC-1 in the linear range of 0.05−940 U/mL, with a detection limit (LOD) of 0.01 U/mL. The immunosensor showed recovery values in the range of 96% for human serum sample analysis, demonstrating its practical applicability.

Item Type:
Journal Article
Journal or Publication Title:
Sensors and Actuators B: Chemical
Additional Information:
This is the author’s version of a work that was accepted for publication in Sensors and Actuators B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators B: Chemical, 330, 2021 DOI: 10.1016/j.snb.2020.129351
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100/3104
Subjects:
ID Code:
151923
Deposited By:
Deposited On:
19 Feb 2021 12:06
Refereed?:
Yes
Published?:
Published
Last Modified:
21 Sep 2021 10:54