Changes in ion selectivity following asymmetrical addition of charge to the selectivity filter of bacterial sodium channels

Fedorenko, Olena and Khovanov, Igor and Roberts, Stephen and Guardiani, Carlo (2020) Changes in ion selectivity following asymmetrical addition of charge to the selectivity filter of bacterial sodium channels. Entropy, 22 (12): 998416. ISSN 1099-4300

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Abstract

Abstract: Voltage-gated sodium channels (NaVs) play fundamental roles in eukaryotes but their exceptional size hinders their structural resolution. Bacterial NaVs are simplified homologues of their eukaryotic counterparts but their use as models of eukaryotic Na+ channels is limited by their homotetrameric structure at odds with the asymmetric Selectivity Filter (SF) of eukaryotic NaVs. This work aims at mimicking the SF of eukaryotic NaVs by engineering radial asymmetry into the SF of bacterial channels. This goal was pursued with two approaches: co-expression of different monomers of the NaChBac bacterial channel to induce the random assembly of heterotetramers, and the concatenation of four bacterial monomers to form a concatemer that can be targeted by site-specific mutagenesis. Patch-clamp measurements and Molecular Dynamics simulations showed that an additional gating charge in the SF leads to a significant increase of Na+ and a modest increase in Ca2+ conductance in the NavMs concatemer in agreement with the behavior of the population of random heterotetramers with the highest proportion of channels with charge −5e. We thus showed that charge, despite being important, is not the only determinant of conduction and selectivity and we created new tools extending the use of bacterial channels as models of eukaryotic counterparts.

Item Type:
Journal Article
Journal or Publication Title:
Entropy
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100/3101
Subjects:
?? physics and astronomy (miscellaneous)statistical and nonlinear physics ??
ID Code:
149733
Deposited By:
Deposited On:
07 Dec 2020 14:00
Refereed?:
Yes
Published?:
Published
Last Modified:
10 Sep 2024 00:35