Design and fabrication of polycaprolactone/gelatin composite scaffolds for diaphragmatic muscle reconstruction

Navaei, Tina and Milan, Peiman and Samadikuchaksaraei, Ali and Davari, Hamid and Hardy, John and Mozafari, Masoud (2021) Design and fabrication of polycaprolactone/gelatin composite scaffolds for diaphragmatic muscle reconstruction. Journal of Tissue Engineering and Regenerative Medicine, 15 (1). pp. 78-87. ISSN 1932-6254

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Abstract

Diaphragmatic wall defects caused by congenital disorders or disease remain a major challenge for physicians worldwide. Polymeric patches have been extensively explored within research laboratories and the clinic for soft tissue and diaphragm reconstruction. However, patch usage may be associated with allergic reaction, infection, granulation, and recurrence of the hernia. In this study, we designed and fabricated a porous scaffold using a combination of 3D printing and freeze-drying techniques. A 3D printed polycaprolactone (PCL) mesh was used to reinforcegelatin scaffolds, representing an advantage over previously reported examples since it provides mechanical strength and flexibility. In vitro studies showed that adherent cells were anchorage-dependent and grew as a monolayer attached to the scaffolds. Microscopic observations indicated better cell attachments for the scaffolds with higher gelatin content as compared with the PCL control samples. Tensile testing demonstrated the mechanical strength of samples was significantly greater than adult diaphragm tissue. The biocompatibility of the specimens was investigated in vivo using a subcutaneous implantation method in BALB/c adult mice for 20 days, with the results indicating superior cellular behavior and attachment on scaffolds containing gelatin in comparison to pure PCL scaffolds, suggesting that the porous PCL/gelatin scaffolds have potential as biodegradable and flexible constructs for diaphragm reconstruction.