Dairy-inspired coatings for bone implants from whey protein isolate-derived self-assembled fibrils

Rabe, Rebecca and Hempel, Ute and Martocq, Laurine and Keppler, Julia K. and Aveyard, Jenny and Douglas, Timothy (2020) Dairy-inspired coatings for bone implants from whey protein isolate-derived self-assembled fibrils. International Journal of Molecular Sciences, 21 (15): 5544. ISSN 1422-0067

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Abstract

To improve integration of a biomaterial with surrounding tissue, its surface properties may be modified by adsorption of biomacromolecules, e.g. fibrils. Whey protein isolate (WPI), a dairy industry by-product, supports osteoblastic cell growth. WPI’s main component, β-lactoglobulin, forms fibrils in acidic solutions. In this study, aiming to develop coatings for biomaterials for bone contact, substrates were coated with WPI fibrils obtained at pH 2 or 3.5. Importantly, WPI fibrils coatings withstood autoclave sterilization and appeared to promote human bone marrow stromal cells (hBMSC) spreading and differentiation. In the future, WPI fibrils coatings could facilitate immobilization of biomolecules with growth stimulating or antimicrobial properties