Sales, Cristina RG and Bernardes Da Silva, Anabela and Carmo-Silva, Elizabete (2020) Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays. UNSPECIFIED, protocols.io.
Full text not available from this repository.Abstract
This collection of protocols includes four assays for measuring Rubisco activity that are used and discussed in the manuscript "Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays" (Sales et al. 2020). Three of the protocols are NADH-linked microtiter plate-based assays for Rubisco activity that use alternative coupling enzymes: 1. GAPDH-GlyPDH, glyceraldehyde-3-phosphate-dehydrogenase and glycerolphosphate-dehydrogenase; 2. PEPC-MDH, phosphoenolpyruvate-carboxylase and malate-dehydrogenase; 3. PK-LDH, pyruvate-kinase and lactate-dehydrogenase. The radiometric assay for Rubisco activity measures the incorporation of 14CO2 into 3-phosphoglycerate (3-PGA). The fifth protocol describes the purification of 2,3-bisphosphate-dependent phosphoglycerate mutase (dPGM), which is required for the NADH-linked microtiter plate-based assays 2 (PEPC-MDH) and 3 (PK-LDH).