Unraveling and manipulating of NADH oxidation by photo-generated holes

Zhang, Shaohua and Shi, Jiafu and Chen, Yixuan and Huo, Qian and Li, Weiran and Wu, Yizhou and Sun, Yiying and Zhang, Yishan and Wang, Xiaodong and Jiang, Zhongyi (2020) Unraveling and manipulating of NADH oxidation by photo-generated holes. ACS Catalysis, 10 (9). pp. 4967-4972. ISSN 2155-5435

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Abstract

Photoenzymatic coupled catalysis, integrating semiconductor photocatalysis and enzymatic catalysis, exhibits great potential for light-driven synthesis. To make photocatalyst and enzyme at play concertedly, nicotinamide-based cofactors have been widely used as electron carrier. However, these cofactors are easily oxidized into enzymatically inactive form by photo-generated holes. Herein, oxidation mechanism of NADH, one typical nicotinamide-based cofactor, by photo-generated holes was reported. With CdS, g-C3N4 and BiVO4 as hole generators, NADH is oxidized into NAD+ or fragmented into ADP-ribose derivatives through multi-step electron transfer. Importantly, fragmentation reaction is inhibited with dopamine and neutral red to coordinate electron transfer between NADH and photo-generated holes.