Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages

Benz, Corinna and Urbaniak, Mick (2020) Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages. In: Trypanosomatids : Methods and Protocols. Methods in Molecular Biology . Humana Press, New York, pp. 125-137. ISBN 9781071602935

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Abstract

Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.