Structural evaluation of human and sheep bone and comparison with synthetic hydroxyapatite by FT‐Raman spectroscopy

Rehman, I. and Smith, R. and Hench, L.L. and Bonfield, W. (1995) Structural evaluation of human and sheep bone and comparison with synthetic hydroxyapatite by FT‐Raman spectroscopy. Journal of Biomedical Materials Research Part A, 29 (10). pp. 1287-1294. ISSN 0021-9304

Abstract

The composition of whole human and sheep cortical bone tissue, and of a synthetic hydroxyapatite (P120), were compared using Fourier transform Raman (FT‐Raman) spectroscopy. Deproteination procedures to remove the bulk of the collagen present in bone tissue allowed isolation of the mineral phase. A comparison of the spectra obtained from both whole and deproteinated bone with those of synthetic hydroxyapatite showed direct correlation only in the region of 952 cm−1 (symmetric PO mode). In contrast, human and sheep bone were very closely matched in both, the organic and inorganic structures. The results demonstrate that deproteination of bone is not a necessary precursor to obtain spectral information. © 1995 John Wiley & Sons, Inc. Copyright © 1995 John Wiley & Sons, Inc.