Confocal immunofluorescence localization of collagen types I, III, IV, V and VI and their ultrastructural organization in term human fetal membranes

Malak, T M and Ockleford, C D and Bell, S C and Dalgleish, R and Bright, N and Macvicar, J (1993) Confocal immunofluorescence localization of collagen types I, III, IV, V and VI and their ultrastructural organization in term human fetal membranes. Placenta, 14 (4). pp. 385-406. ISSN 0143-4004

Full text not available from this repository.

Abstract

The distribution of collagen types I, III, IV, V and VI in term human fetal membranes was examined using conventional and confocal indirect immunofluorescence techniques. Collagens I and III were present in most of the layers of fetal membranes except in the trophoblast layer contrary to what has been previously reported. Although collagen IV is considered to be a basement membrane component our study, using monoclonal and polyclonal antibodies, showed its consistent presence in the spongy and reticular layers in high intensity. This was first report on the distribution of type V collagen in the chorion where it was found in the reticular and in the trophoblast layers. Type VI collagen was present mainly in the amnion and the reticular layer. The ultrastructural examination of the extracellular matrix showed that the main fibrous skeleton of the fetal membranes was formed of large banded fibres (Ultrastructurally identical to collagens types I and III) connected together and to the epithelial basement membranes by networks of unbanded filaments (collagen types V, VI and other components). The extensive and continuous networks formed by these collagens may be a major factor responsible for the mechanical integrity of the fetal membranes.

Item Type:
Journal Article
Journal or Publication Title:
Placenta
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2743
Subjects:
?? CHEMISTRYFEMALEFLUORESCENT ANTIBODY TECHNIQUEHUMANSPREGNANCYDEVELOPMENTAL BIOLOGYOBSTETRICS AND GYNAECOLOGYREPRODUCTIVE MEDICINE ??
ID Code:
130790
Deposited By:
Deposited On:
28 Jan 2019 10:40
Refereed?:
Yes
Published?:
Published
Last Modified:
20 Sep 2023 01:19