Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera

Yang, X. and Xu, P. and Yuan, H. and Graham, R.I. and Wilson, Kenneth and Wu, K. (2019) Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera. Journal of Invertebrate Pathology, 160. pp. 1-7. ISSN 0022-2011

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We characterize a novel picorna-like virus, named Helicoverpa armigera Nora virus (HaNV), with a genome length of 11,200 nts, the sequence of which was isolated from the lepidopteran host cotton bollworm Helicoverpa armigera, using RNA-Seq. Phylogenetic analysis, using the putative amino acid sequence of the conserved RNA-dependent RNA polymerase (RdRp) domain, indicated that HaNV clustered with Spodoptera exigua Nora virus, Drosophila Nora virus and Nasonia vitripennis virus-3 with a high bootstrap value (100%), which might indicate a new viral family within the order Picornavirales. HaNV was efficiently horizontally transmitted between hosts via contaminated food, and transmission was found to be dose-dependent (up to 100% efficiency with 109 viral copy number/µl). HaNV was also found to be transmitted vertically from parent to offspring, mainly through transovum transmission (virus contamination on the surface of the eggs), but having a lower transmission efficiency (around 43%). Infection distribution within the host was also investigated, with HaNV mainly found in only the gut of both adult moths and larvae (>90%). Moreover, our results showed that HaNV appears not to be an overtly pathogenic virus to its host.

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Journal Article
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Journal of Invertebrate Pathology
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This is the author’s version of a work that was accepted for publication in Journal of Invertebrate Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Invertebrate Pathology, 160, 2019 DOI: 10.1016/j.jip.2018.011.003
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10 Jan 2019 10:06
Last Modified:
15 Sep 2023 00:50