Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein

Li, Jihong and Meng, Chunchun and Ren, Tingting and Wang, Wei and Zhang, Yaodan and Yuan, Weifeng and Xu, Shuqin and Sun, Yingjie and Tan, Lei and Song, Cuiping and Liao, Ying and Nair, Venugopal and Munir, Muhammad and Ding, Zhuang and Liu, Xiufan and Qiu, Xusheng and Ding, Chan (2018) Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein. Journal of Virological Methods, 260. pp. 88-97. ISSN 0166-0934

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Abstract

Newcastle disease virus (NDV) V protein is crucial for viral interferon (IFN) antagonism and virulence, determining its host range restriction. However, little information is available on the B cell epitopes of V protein and the subcellular movement of V protein in the process of NDV infection. In this study, the monoclonal antibody (mAb) clone 3D7 against genotype VII NDV V protein was generated by immunizing mice with a purified recombinant His-tagged carboxyl-terminal domain (CTD) region of V protein. Fine epitope mapping analysis and B-cell epitope prediction indicated that mAb 3D7 recognized a linear epitope 152RGPAELWK159, which is located in the V protein CTD region. Sequence alignment showed that the mAb clone 3D7-recognized epitope is highly conserved among Class II genotype VII NDV strains, but not among other genotypes, suggesting it could serve as a genetic marker to differentiate NDV genotypes. Furthermore, the movement of V protein during NDV replication in infected cells were determined by using this mAb. It was found that V protein localized around the nucleus during virus replication. The establishment of V protein-specific mAb and identification of its epitope extend our understanding of the antigenic characteristics of V protein and provide a basis for the development of epitope-based diagnostic assays.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Virological Methods
Additional Information:
This is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 260, 2018 DOI: 10.1016/j.jviromet.2018.07.009
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2406
Subjects:
ID Code:
126715
Deposited By:
Deposited On:
06 Aug 2018 12:40
Refereed?:
Yes
Published?:
Published
Last Modified:
24 Nov 2020 06:34