Li, Jihong and Meng, Chunchun and Ren, Tingting and Wang, Wei and Zhang, Yaodan and Yuan, Weifeng and Xu, Shuqin and Sun, Yingjie and Tan, Lei and Song, Cuiping and Liao, Ying and Nair, Venugopal and Munir, Muhammad and Ding, Zhuang and Liu, Xiufan and Qiu, Xusheng and Ding, Chan (2018) Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein. Journal of Virological Methods, 260. pp. 88-97. ISSN 0166-0934
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Abstract
Newcastle disease virus (NDV) V protein is crucial for viral interferon (IFN) antagonism and virulence, determining its host range restriction. However, little information is available on the B cell epitopes of V protein and the subcellular movement of V protein in the process of NDV infection. In this study, the monoclonal antibody (mAb) clone 3D7 against genotype VII NDV V protein was generated by immunizing mice with a purified recombinant His-tagged carboxyl-terminal domain (CTD) region of V protein. Fine epitope mapping analysis and B-cell epitope prediction indicated that mAb 3D7 recognized a linear epitope 152RGPAELWK159, which is located in the V protein CTD region. Sequence alignment showed that the mAb clone 3D7-recognized epitope is highly conserved among Class II genotype VII NDV strains, but not among other genotypes, suggesting it could serve as a genetic marker to differentiate NDV genotypes. Furthermore, the movement of V protein during NDV replication in infected cells were determined by using this mAb. It was found that V protein localized around the nucleus during virus replication. The establishment of V protein-specific mAb and identification of its epitope extend our understanding of the antigenic characteristics of V protein and provide a basis for the development of epitope-based diagnostic assays.