Azevedo, Ricardo A. and Damerval, Catherine and Lea, Peter J. and Landry, Jacques and Bellato, Cláudia M. and Meinhardt, Lyndel W. and Le Guilloux, Martine and Delhaye, Sonia and Toro, Alejandro A. and Gaziola, Salete A. and Varisi, Vanderlei A. and Gratão, Priscila Lupino (2004) Genetic control of lysine metabolism in maize endosperm mutants. Functional Plant Biology, 31 (4). pp. 339-348. ISSN 1445-4408
Full text not available from this repository.Abstract
The capacity of three maize endosperm opaque mutants (o10, o11 and o13) to accumulate soluble lysine in the seed in relation to their wildtype counterpart, W22+, was investigated. The W22o13 and W22o11 mutants exhibited 278% and 186% increases in soluble lysine, respectively, while for W22o10, a 36% decrease was observed, compared with the wildtype. A quantitative and qualitative study of the N constituents of the endosperm has been conducted and data obtained for the total protein, non-protein N, soluble amino acids, albumins / globulins, zeins and glutelins present in the seed of the mutants. Following 2D–PAGE, a total of 38 different forms of zein polypeptides were detected and considerable differences were noted between the three mutant lines. The metabolism of lysine was also studied by analysis of the enzymes aspartate kinase, homoserine dehydrogenase, lysine 2-oxoglutarate reductase and saccharopine dehydrogenase, which exhibited major changes in activity, depending on the genotype, suggesting that the mutant genes may have distinct regulatory activities.