Holtman, Wessel L. and Roberts, Michael R. and Oppedijk, Berry J. and Testerink, Christa and Van Zeijl, Mieke J. and Wang, Mei (2000) 14-3-3 proteins intact with a 13-lipoxygenase, but not with a 9-lipoxygenase. FEBS Letters, 474 (1). pp. 48-52. ISSN 0014-5793Full text not available from this repository.
Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.
|Journal or Publication Title:||FEBS Letters|
|Uncontrolled Keywords:||14-3-3 protein ; Lipoxygenase ; Interaction|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Dr Michael R Roberts|
|Deposited On:||11 Jun 2008 16:47|
|Last Modified:||26 Jul 2012 18:37|
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