Lauder, R. M. and Huckerby, T. N. and Nieduszynski, I. A. (1994) Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion. The Biochemical journal, 302 (P. pp. 417-423.
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Abstract
The structure of the repeat region and chain caps of the N-linked keratan sulphate chains attached to bovine tracheal cartilage fibromodulin has been examined. The chains were fragmented by keratanase digestion, the resultant oligosaccharides isolated by strong anion-exchange chromatography, and their structures determined using high-field 1H-n.m.r. spectroscopy. The chains were found to possess the following general structure: [formula: see text] All of the capping oligosaccharides isolated terminate with alpha(2-3)-linked N-acetylneuraminic acid. No alpha(2-6)-linked N-acetylneuraminic acid chain terminators, nor any fucose, alpha (1-3)-linked to N-acetylglucosamine along the repeat region, were detected. This work demonstrates that the structure of the repeat region and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage is identical with that of O-linked keratan sulphate chains attached to aggrecan derived from non-articular cartilage.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | The Biochemical journal |
| Subjects: | R Medicine > R Medicine (General) |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 34798 |
| Deposited By: | Dr Bob Lauder |
| Deposited On: | 08 Dec 2010 14:49 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 17:41 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/34798 |
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