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Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion.

Lauder, R. M. and Huckerby, T. N. and Nieduszynski, I. A. (1994) Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion. Biochemical Journal, 302. pp. 417-423. ISSN 0264-6021

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    Abstract

    The structure of the repeat region and chain caps of the N-linked keratan sulphate chains attached to bovine tracheal cartilage fibromodulin has been examined. The chains were fragmented by keratanase digestion, the resultant oligosaccharides isolated by strong anion-exchange chromatography, and their structures determined using high-field 1H-n.m.r. spectroscopy. The chains were found to possess the following general structure: [formula: see text] All of the capping oligosaccharides isolated terminate with alpha(2-3)-linked N-acetylneuraminic acid. No alpha(2-6)-linked N-acetylneuraminic acid chain terminators, nor any fucose, alpha (1-3)-linked to N-acetylglucosamine along the repeat region, were detected. This work demonstrates that the structure of the repeat region and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage is identical with that of O-linked keratan sulphate chains attached to aggrecan derived from non-articular cartilage.

    Item Type: Article
    Journal or Publication Title: Biochemical Journal
    Subjects: R Medicine > R Medicine (General)
    Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
    Faculty of Science and Technology > Lancaster Environment Centre
    ID Code: 34798
    Deposited By: Dr Bob Lauder
    Deposited On: 08 Dec 2010 14:49
    Refereed?: Yes
    Published?: Published
    Last Modified: 17 Sep 2013 08:21
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/34798

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