Kuznetsov, Konstantin and Stepanova, Alena and Kvon, Ren and Douglas, Timothy Edward Lim and Kuznetsov, Nikita and Chernonosova, Vera and Zaporozhchenko, Ivan and Kharkova, Maria and Romanova, Irina and Karpenko, Andrey and Laktionov, Pavel (2018) Electrospun Produced 3D Matrices for Covering of Vascular Stents : Paclitaxel Release Depending on Fiber Structure and Composition of the External Environment. Materials, 11 (11): 2176. ISSN 1996-1944
preprints201810.0231.v1.pdf - Accepted Version
Available under License Creative Commons Attribution.
Download (611kB)
Abstract
Paclitaxel is a natural, highly lipophilic anti proliferative drug widely used in medicine. We have studied the release of tritium-labeled paclitaxel (3H-PTX) from matrices destined for the coating of vascular stents and produced by the electrospinning method from the solutions of polycaprolactone (PCL) with paclitaxel (PTX) in hexafluoisopropanol (HFIP) and/or solutions of PCL with PTX and human serum albumin (HSA) in HFIP or HIFP-dimethyl sulphoxide (DMSO) blend. The release of PTX has been shown to depend on the composition of electrospinning solution, as well as the surrounding medium, particularly the concentration of free PTX and PTX-binding biomolecules present in human serum. It was shown that 3D matrices can completely release PTX without weight loss. Two-phase PTX release from optimized 3D matrices was obtained: ~27% of PTX was released in the first day, another 8% were released over the next 26 days. Wherein ~2.8%, ~2.3%, and ~0.25% of PTX was released on day 3, 9, and 27, respectively. Considering PTX toxicity, the rate of its diffusion through the arterial wall, and the data obtained the minimum cytostatic dose of the drug in the arterial wall will be maintained for at least three months. © 2018 by the authors.