Identification of a proctolin preprohormone gene (Proct) of Drosophila melanogaster: Expression and predicted prohormone processing

Taylor, C. A. M. and Winther, A. M. E. and Siviter, R. J. and Shirras, A. D. and Isaac, R. E. and Nassel, D. R. (2004) Identification of a proctolin preprohormone gene (Proct) of Drosophila melanogaster: Expression and predicted prohormone processing. Journal of Neurobiology, 58 (3). pp. 379-391. ISSN 0022-3034

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Abstract

Proctolin was the first insect neuropeptide to be sequenced and has been the subject of many physiological and pharmacological studies in insects and crustaceans. We have identified a Drosophila gene (CG7105, Proct) encoding a precursor protein containing the neuropeptide proctolin (RYLPT). In situ hybridization with a riboprobe to the Proct gene revealed a distribution of transcript in neurons of the larval central nervous system (CNS) matching that seen with antiserum to proctolin. An antiserum raised to a sequence in the precursor downstream of proctolin labeled the same neurons as those seen with the antiproctolin antisera. The predicted protein encoded by Proct has a single copy of the RYLPT sequence that directly follows the predicted signal peptidase cleavage point and precedes a consensus recognition site for a furinlike processing endoprotease. Ectopic expression of Proct in the CNS and midgut via the GAL4-UAS system, using an Actin5C-GAL4 driver, confirmed that the predicted preproproctolin can be processed to generate immunoreactive proctolin peptide. Pupae over-expressing Proct displayed a 14% increase in heart rate, providing evidence in support of a cardioacceleratory endocrine function for proctolin in Drosophila. The distribution of proctolin suggests roles as a neuromodulator in motoneurons and interneurons, and as a neurohormone that could be released from brain neurosecretory cells with terminations in the ring gland. (C) 2003 Wiley Periodicals, Inc. J Neurobiol 58: 379-391, 2004.

Item Type: Journal Article
Journal or Publication Title: Journal of Neurobiology
Uncontrolled Keywords: /dk/atira/pure/researchoutput/libraryofcongress/qh301
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 9344
Deposited By: Dr Alan Shirras
Deposited On: 05 Jun 2008 10:44
Refereed?: Yes
Published?: Published
Last Modified: 19 Aug 2019 00:39
URI: https://eprints.lancs.ac.uk/id/eprint/9344

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