Phospholiphase C is required for the control of stomatal aperture by ABA.

Hunt, L. and Mills, L. N. and Pical, C. and Leckie, C. P. and Aitken, F. L. and Kopka, J. and Mueller-Roeber, B. and McAinsh, Martin and Hetherington, A. M. and Gray, J. E. (2003) Phospholiphase C is required for the control of stomatal aperture by ABA. Plant Journal, 34 (1). pp. 47-55. ISSN 0960-7412

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The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.

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Journal Article
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Plant Journal
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20 May 2008 10:49
Last Modified:
14 Sep 2023 23:52