Application of whey protein isolate in bone regeneration : Effects on growth and osteogenic differentiation of bone-forming cells

Douglas, Timothy E.L. and Vandrovcová, Marta and Kročilová, Nikola and Keppler, Julia K. and Zárubová, Jana and Skirtach, Andre G. and Bačáková, Lucie (2018) Application of whey protein isolate in bone regeneration : Effects on growth and osteogenic differentiation of bone-forming cells. Journal of Dairy Science, 101 (1). pp. 28-36. ISSN 0022-0302

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Recently, milk-derived proteins have attracted attention for applications in the biomedical field such as tissue regeneration. Whey protein isolate (WPI), especially its main component β-lactoglobulin, can modulate immunity and acts as an antioxidant, antitumor, antiviral, and antibacterial agent. There are very few reports of the application of WPI in tissue engineering, especially in bone tissue engineering. In this study, we tested the influence of different concentrations of WPI on behavior of human osteoblast-like Saos-2 cells, human adipose tissue-derived stem cells (ASC), and human neonatal dermal fibroblasts (FIB). The positive effect on growth was apparent for Saos-2 cells and FIB but not for ASC. However, the expression of markers characteristic for early osteogenic cell differentiation [type-I collagen (COL1) and alkaline phosphatase (ALP)] as well as ALP activity, increased dose-dependently in ASC. Importantly, Saos-2 cells were able to deposit calcium in the presence of WPI, even in a proliferation medium without other supplements that support osteogenic cell differentiation. The results indicate that, depending on the cell type, WPI can act as an enhancer of cell proliferation and osteogenic differentiation. Therefore, enrichment of biomaterials for bone regeneration with WPI seems a promising approach, especially due to the low cost of WPI.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Dairy Science
Additional Information:
This is the author’s version of a work that was accepted for publication in Journal of Dairy Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Dairy Science, 101, 1, 2018 DOI: 10.3168/jds.2017-13119
Uncontrolled Keywords:
?? whey protein isolatecell proliferationosteogenic differentiationadipose-derived stem cellgeneticsfood scienceanimal science and zoology ??
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Deposited On:
05 Jan 2018 14:00
Last Modified:
31 Dec 2023 00:52