Oxygen effect for double-strand break induction determined by pulsed-field gel electrophoresis.

Whitaker, Stephen J. and McMillan, Trevor J. (1992) Oxygen effect for double-strand break induction determined by pulsed-field gel electrophoresis. International Journal of Radiation Biology, 61 (1). pp. 29-41. ISSN 0955-3002

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Abstract

The induction of DNA double-strand breaks (dsb) following irradiation under oxygenated and hypoxic conditions with and without misonidazole was measured by pulsed-field gel electrophoresis (PFGE) in a human bladder carcinoma cell line. The dose-response curve for DNA dsb detection by PFGE was biphasic with an apparent reduction in rate of dsb induced with dose. Oxygen enhancement ratios (OER) for cell survival (at a surviving fraction of 0·1) and for DNA damage assessed by PFGE (at 80% retained) were 2·0 and 3·0 respectively. Dose-modifying factors for misonidazole (15 mm), of 1·9 (survival) and 2·4 (DNA damage) were found. Although the magnitude of the inter-experiment variations limit the precision with which cell survival and DNA electrophoresis can be compared, the data do support a simple correlation between these two measures of response. When DNA dsb induction frequency was assessed from the number average molecular weight, values of 2·7 (±0·3), 0·7 (±0·1) and 2·6 (±0·5) × 10-9 dsb/bp/Gy were found for irradiation under oxic, hypoxic alone and hypoxic + misonidazole conditions respectively. This gives an OER of 3·9 and a DMF of 3·7.

Item Type:
Journal Article
Journal or Publication Title:
International Journal of Radiation Biology
Uncontrolled Keywords:
/dk/atira/pure/researchoutput/libraryofcongress/qh301
Subjects:
ID Code:
8740
Deposited On:
12 May 2008 15:08
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 07:15