Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins

Harmer, Jane and Qi, Xin and Toniolo, Gabriella and Patel, Aysha and Shaw, Hannah and Benson, Fiona E. and Ginger, Michael L. and McKean, Paul G. (2017) Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins. Protist, 168 (4). pp. 452-466. ISSN 1434-4610

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Abstract

TOF-LisH-PLL motifs defines FOP family proteins; some members are involved in flagellum assembly. The critical role of FOP family protein FOR20 is poorly understood. Here, we report relative localisations of the four FOP family proteins in parasitic Trypanosoma brucei: TbRP2, TbOFD1 and TbFOP/FOP1-like are mature basal body proteins whereas TbFOR20 is present on pro- and mature basal bodies − on the latter it localises distal to TbRP2. We discuss how the data, together with published work for another protist Giardia intestinalis, informs on likely FOR20 function. Moreover, our localisation study provides convincing evidence that the antigen recognised by monoclonal antibody YL1/2 at trypanosome mature basal bodies is FOP family protein TbRP2, not tyrosinated α-tubulin as widely stated in the literature. Curiously, FOR20 proteins from T. brucei and closely related African trypanosomes possess short, negatively-charged N-terminal extensions absent from FOR20 in other trypanosomatids and other eukaryotes. The extension is necessary for protein targeting, but insufficient to re-direct TbRP2 to probasal bodies. Yet, FOR20 from the American trypanosome T. cruzi, which lacks any extension, localises to pro- and mature basal bodies when expressed in T. brucei. This identifies unexpected variation in FOR20 architecture that is presently unique to one clade of trypanosomatids.

Item Type:
Journal Article
Journal or Publication Title:
Protist
Additional Information:
This is the author’s version of a work that was accepted for publication in Protist. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Protist, 168, (4), 2017 DOI: 10.1016/j.protis.2017.07.002
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2404
Subjects:
ID Code:
87086
Deposited By:
Deposited On:
17 Jul 2017 15:40
Refereed?:
Yes
Published?:
Published
Last Modified:
12 Jul 2020 06:41