A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples

Jiang, Bo and Li, Guanghe and Xing, Yi and Zhang, Dayi and Jia, Jianli and Cui, Zhisong and Luan, Xiao and Tang, Hui (2017) A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples. Chemosphere, 184. pp. 384-392. ISSN 0045-6535

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Abstract

Whole-cell bioreporters have emerged as promising tools for genotoxicity evaluation, due to their rapidity, cost-effectiveness, sensitivity and selectivity. In this study, a method for detecting genotoxicity in environmental samples was developed using the bioluminescent whole-cell bioreporter Escherichia coli recA::luxCDABE. To further test its performance in a real world scenario, the E. coli bioreporter was applied in two cases: i) soil samples collected from chromium(VI) contaminated sites; ii) crude oil contaminated seawater collected after the Jiaozhou Bay oil spill which occurred in 2013. The chromium(VI) contaminated soils were pretreated by water extraction, and directly exposed to the bioreporter in two phases: aqueous soil extraction (water phase) and soil supernatant (solid phase). The results indicated that both extractable and soil particle fixed chromium(VI) were bioavailable to the bioreporter, and the solid-phase contact bioreporter assay provided a more precise evaluation of soil genotoxicity. For crude oil contaminated seawater, the response of the bioreporter clearly illustrated the spatial and time change in genotoxicity surrounding the spill site, suggesting that the crude oil degradation process decreased the genotoxic risk to ecosystem. In addition, the performance of the bioreporter was simulated by a modified cross-regulation gene expression model, which quantitatively described the DNA damage response of the E. coli bioreporter. Accordingly, the bioluminescent response of the bioreporter was calculated as the mitomycin C equivalent, enabling quantitative comparison of genotoxicities between different environmental samples. This bioreporter assay provides a rapid and sensitive screening tool for direct genotoxicity assessment of environmental samples.

Item Type:
Journal Article
Journal or Publication Title:
Chemosphere
Additional Information:
This is the author’s version of a work that was accepted for publication in Chemosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemosphere, 184, 2017 DOI: 10.1016/j.chemosphere.2017.05.159
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1600
Subjects:
ID Code:
86649
Deposited By:
Deposited On:
09 Jun 2017 12:54
Refereed?:
Yes
Published?:
Published
Last Modified:
24 Nov 2020 05:19