Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes

Robinson, Nicholas P. and Bell, Stephen D. (2007) Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes. Proceedings of the National Academy of Sciences of the United States of America, 104 (14). pp. 5806-5811. ISSN 0027-8424

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Abstract

In all three domains of life, DNA replication begins at specialized loci termed replication origins. In bacteria, replication initiates from a single, clearly defined site. In contrast, eukaryotic organisms exploit a multitude of replication origins, dividing their genomes into an array of short contiguous units. Recently, the multiple replication origin paradigm has also been demonstrated within the archaeal domain of life, with the discovery that the hyperthermophilic archaeon Sulfolobus has three replication origins. However, the evolutionary mechanism driving the progression from single to multiple origin usage remains unclear. Here, we demonstrate that Aeropyrum pernix, a distant relative of Sulfolobus, has two origins. Comparison with the Sulfolobus origins provides evidence for evolution of replicon complexity by capture of extrachromosomal genetic elements. We additionally identify a previously unrecognized candidate archaeal initiator protein that is distantly related to eukaryotic Cdt1. Our data thus provide evidence that horizontal gene transfer, in addition to its well-established role in contributing to the information content of chromosomes, may fundamentally alter the manner in which the host chromosome is replicated.

Item Type:
Journal Article
Journal or Publication Title:
Proceedings of the National Academy of Sciences of the United States of America
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1000
Subjects:
ID Code:
86206
Deposited By:
Deposited On:
04 May 2017 13:20
Refereed?:
Yes
Published?:
Published
Last Modified:
11 Jun 2020 05:36