Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus

Robinson, Nicholas P. and Dionne, Isabelle and Lundgren, Magnus and Marsh, Victoria L. and Bernander, Rolf and Bell, Stephen D. (2004) Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus. Cell, 116 (1). pp. 25-38. ISSN 0092-8674

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Abstract

Eukaryotic chromosomes possess multiple origins of replication, whereas bacterial chromosomes are replicated from a single origin. The archaeon Pyrococcus abyssi also appears to have a single origin, suggesting a common rule for prokaryotes. However, in the current work, we describe the identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus. Further, we identify conserved sequence motifs within the origins that are recognized by a family of three Sulfolobus proteins that are homologous to the eukaryotic initiator proteins Orc1 and Cdc6. We demonstrate that the two origins are recognized by distinct subsets of these Orc1/Cdc6 homologs. These data, in conjunction with an analysis of the levels of the three Orc1/Cdc6 proteins in different growth phases and cell cycle stages, lead us to propose a model for the roles for these proteins in modulating origin activity.

Item Type: Journal Article
Journal or Publication Title: Cell
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1300
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 86201
Deposited By: ep_importer_pure
Deposited On: 04 May 2017 13:06
Refereed?: Yes
Published?: Published
Last Modified: 22 Jun 2019 08:02
URI: https://eprints.lancs.ac.uk/id/eprint/86201

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