Enhancing soybean photosynthetic CO2 assimilation using a cyanobacterial membrane protein, ictB

Hay, William T. and Bihmidine, Saadia and Mutlu, Nedim and Hoang, Khang Le and Awada, Tala and Weeks, Donald P. and Clemente, Tom E. and Long, Stephen P. (2017) Enhancing soybean photosynthetic CO2 assimilation using a cyanobacterial membrane protein, ictB. Journal of Plant Physiology, 212. pp. 58-68. ISSN 0176-1617

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Abstract

Abstract Soybean C3 photosynthesis can suffer a severe loss in efficiency due to photorespiration and the lack of a carbon concentrating mechanism (CCM) such as those present in other plant species or cyanobacteria. Transgenic soybean (Glycine max cv. Thorne) plants constitutively expressing cyanobacterial ictB (inorganic carbon transporter B) gene were generated using Agrobacterium-mediated transformation. Although more recent data suggest that ictB does not actively transport HCO3-/CO2, there is nevertheless mounting evidence that transformation with this gene can increase higher plant photosynthesis. The hypothesis that expression of the ictB gene would improve photosynthesis, biomass production and seed yield in soybean was tested, in two independent replicated greenhouse and field trials. Results showed significant increases in photosynthetic CO2 uptake (Anet) and dry mass in transgenic relative to wild type (WT) control plants in both the greenhouse and field trials. Transgenic plants also showed increased photosynthetic rates and biomass production during a drought mimic study. The findings presented herein demonstrate that ictB, as a single-gene, contributes to enhancement in various yield parameters in a major commodity crop and point to the significant role that biotechnological approaches to increasing photosynthetic efficiency can play in helping to meet increased global demands for food.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Plant Physiology
Additional Information:
This is the author’s version of a work that was accepted for publication in Journal of Plant Physiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Plant Physiology, 212, 2017 DOI: 10.1016/j.jplph.2017.02.003
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1314
Subjects:
ID Code:
84809
Deposited By:
Deposited On:
21 Feb 2017 14:36
Refereed?:
Yes
Published?:
Published
Last Modified:
09 Aug 2020 04:58