Sodium binding sites and permeation mechanism in the NaChBac channel:a molecular dynamics study

Guardiani, Carlo and Rodger, P. Mark and Fedorenko, Olena and Roberts, Stephen Kenneth and Khovanov, Igor A. (2017) Sodium binding sites and permeation mechanism in the NaChBac channel:a molecular dynamics study. Journal of Chemical Theory and Computation, 13 (3). pp. 1389-1400. ISSN 1549-9618

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Abstract

NaChBac was the first discovered bacterial sodium voltage-dependent channel, yet computational studies are still limited due to the lack of a crystal structure. In this work, a pore-only construct built using the NavMs template was investigated using unbiased molecular dynamics and metadynamics. The potential of mean force (PMF) from the unbiased run features four minima, three of which correspond to sites IN, CEN, and HFS discovered in NavAb. During the run, the selectivity filter (SF) is spontaneously occupied by two ions, and frequent access of a third one is often observed. In the innermost sites IN and CEN, Na+ is fully hydrated by six water molecules and occupies an on-axis position. In site HFS sodium interacts with a glutamate and a serine from the same subunit and is forced to adopt an off-axis placement. Metadynamics simulations biasing one and two ions show an energy barrier in the SF that prevents single-ion permeation. An analysis of the permeation mechanism was performed both computing minimum energy paths in the axial–axial PMF and through a combination of Markov state modeling and transition path theory. Both approaches reveal a knock-on mechanism involving at least two but possibly three ions. The currents predicted from the unbiased simulation using linear response theory are in excellent agreement with single-channel patch-clamp recordings

Item Type: Journal Article
Journal or Publication Title: Journal of Chemical Theory and Computation
Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in JournaL of Chemical Theory and Computation, copyright ©2016 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.jctc.6b01035
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1700/1706
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 84667
Deposited By: ep_importer_pure
Deposited On: 14 Feb 2017 08:54
Refereed?: Yes
Published?: Published
Last Modified: 21 Feb 2020 03:30
URI: https://eprints.lancs.ac.uk/id/eprint/84667

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