Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

Yuan, He and Xu, Pengjun and [Unknown], XianmingYang and Graham, Robert I. and Wilson, Kenneth and Wu, Kongming (2017) Characterization of a novel member of genus Iflavirus in Helicoverpa armigera. Journal of Invertebrate Pathology, 144. pp. 65-73. ISSN 0022-2011

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The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3,021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 108 copies /μl) while vertical transmission efficiency was found to be relatively low (< 28.57%). These results suggest that we have found a novel member of genus Iflavirus in H. armigera.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Invertebrate Pathology
Additional Information:
This is the author’s version of a work that was accepted for publication in Journal of Invertebrate Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Invertebrate Pathology, 144, 2017 DOI: 10.1016/j.jip.2017.01.011
Uncontrolled Keywords:
?? helicoverpa armigeraiflavirusrna virushorizontal transmissionvertical transmissiontissue distributionecology, evolution, behavior and systematics ??
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Deposited On:
08 Feb 2017 15:18
Last Modified:
31 Dec 2023 00:47