Ionic Coulomb blockade and anomalous mole fraction effect in NaChBac bacterial ion channels

Kaufman, I. Kh and Fedorenko, O. A. and Luchinsky, D. G. and Gibby, W. A. T. and Roberts, Stephen and McClintock, Peter Vaughan Elsmere and Eisenberg, R. S. (2016) Ionic Coulomb blockade and anomalous mole fraction effect in NaChBac bacterial ion channels. arXiv.

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Abstract

We report an experimental study of the influences of the fixed charge and bulk ionic concentrations on the conduction of biological ion channels, and we consider the results within the framework of the ionic Coulomb blockade model of permeation and selectivity. Voltage clamp recordings were used to investigate the Na$^+$/Ca$^{2+}$ anomalous mole fraction effect (AMFE) exhibited by the bacterial sodium channel NaChBac and its mutants. Site-directed mutagenesis was used to study the effect of either increasing or decreasing the fixed charge in their selectivity filters for comparison with the predictions of the Coulomb blockade model. The model was found to describe well some aspects of the experimental (divalent blockade and AMFE) and simulated (discrete multi-ion conduction and occupancy band) phenomena, including a concentration-dependent shift of the Coulomb staircase. These results substantially extend the understanding of ion channel selectivity and may also be applicable to biomimetic nanopores with charged walls.

Item Type: Journal Article
Journal or Publication Title: arXiv
Additional Information: 12 pages, 5 figures, 32 references, submitted to EPJ
Subjects:
Departments: Faculty of Science and Technology > Physics
Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 83968
Deposited By: ep_importer_pure
Deposited On: 13 Jan 2017 09:22
Refereed?: No
Published?: Published
Last Modified: 21 Oct 2019 00:56
URI: https://eprints.lancs.ac.uk/id/eprint/83968

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