Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes

Ivanova, Irina M. and Nepogodiev, Sergey A. and Saalbach, Gerhard and O'Neill, Ellis C. and Urbaniak, Michael D. and Ferguson, Michael A.J. and Gurcha, Sudagar S. and Besra, Gurdyal S. and Field, Robert A. (2017) Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes. Carbohydrate Research, 438. pp. 26-38. ISSN 0008-6215

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Abstract

Synthetic hexynyl α-D-mannopyranoside and its α-1,6-linked disaccharide counterpart were fluorescently labelled through CuAAC click chemistry with 3-azido-7-hydroxycoumarin. The resulting triazolyl-coumarin adducts, which were amenable to analysis by TLC, HPLC and mass spectrometry, proved to be acceptor substrates for α-1,6-ManT activities in mycobacterial membranes, as well as α- and β-GalT activities in trypanosomal membranes, benchmarking the potential of the fluorescent acceptor approach against earlier radiochemical assays. Following on to explore the glycobiology of the benign protozoan alga Euglena gracilis, α-1,3- and α-1,2-ManT activities were detected in membrane preparations, along with GlcT, Glc-P-T and GlcNAc-P-T activities. These studies serve to demonstrate the potential of readily accessible fluorescent glycans as substrates for exploring carbohydrate active enzymes.

Item Type:
Journal Article
Journal or Publication Title:
Carbohydrate Research
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1600/1602
Subjects:
ID Code:
83372
Deposited By:
Deposited On:
02 Dec 2016 11:36
Refereed?:
Yes
Published?:
Published
Last Modified:
23 Sep 2020 03:13