Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis

Atkinson, Nicky and Leitão, Nuno and Orr, Douglas John and Meyer, Moritz and Carmo-Silva, Ana Elizabete and Griffiths, Howard and Smith, Alison and McCormick, Alistair (2017) Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytologist, 214 (2). pp. 655-667. ISSN 0028-646X

[img]
Preview
PDF (McCormick_text.R14)
McCormick_text.R14.pdf - Accepted Version
Available under License Creative Commons Attribution-NonCommercial.

Download (688kB)

Abstract

Introducing components of algal carbon concentrating mechanisms (CCMs) into higher plant chloroplasts could increase photosynthetic productivity. A key component is the Rubisco-containing pyrenoid that is needed to minimise CO2 retro-diffusion for CCM operating efficiency. Rubisco in Arabidopsis was re-engineered to incorporate sequence elements that are thought to be essential for recruitment of Rubisco to the pyrenoid, namely the algal Rubisco small subunit (SSU, encoded by rbcS) or only the surface-exposed algal SSU α-helices. Leaves of Arabidopsis rbcs mutants expressing ‘pyrenoid-competent’ chimeric Arabidopsis SSUs containing the SSU α-helices from Chlamydomonas reinhardtii can form hybrid Rubisco complexes with catalytic properties similar to those of native Rubisco, suggesting that the α-helices are catalytically neutral. The growth and photosynthetic performance of complemented Arabidopsis rbcs mutants producing near wild-type levels of the hybrid Rubisco were similar to those of wild-type controls. Arabidopsis rbcs mutants expressing a Chlamydomonas SSU differed from wild-type plants with respect to Rubisco catalysis, photosynthesis and growth. This confirms a role for the SSU in influencing Rubisco catalytic properties.

Item Type:
Journal Article
Journal or Publication Title:
New Phytologist
Additional Information:
This is the peer reviewed version of the following article: Atkinson, N., Leitão, N., Orr, D. J., Meyer, M. T., Carmo-Silva, E., Griffiths, H., Smith, A. M. and McCormick, A. J. (2017), Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis. New Phytol, 214: 655–667. doi:10.1111/nph.14414 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.14414/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700
Subjects:
ID Code:
83158
Deposited By:
Deposited On:
25 Nov 2016 09:52
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Dec 2020 04:13