Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium

Song, Mengke and Yang, Ying and Jiang, Longfei and Hong, Qing and Zhang, Dayi and Shen, Zhenguo and Yin, Hua and Luo, Chunling (2017) Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium. Environmental Pollution, 220 (Part B). pp. 1059-1067. ISSN 0269-7491

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Abstract

A copper-tolerant phenanthrene (PHE)-degrading bacterium, strain Sphingobium sp. PHE-1, was newly isolated from the activated sludge in a wastewater treatment plant. Two key genes, ahdA1b-1 encoding polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHDɑ) and xyLE encoding catechol-2,3-dioxygenase (C23O), involved in the PHE metabolism by strain PHE-1 were identified. The PAH-RHD gene cluster showed 96% identity with the same cluster of Sphingomonas sp. P2. Our results indicated the induced transcription of xylE and ahdA1b-1 genes by PHE, simultaneously promoted by Cu(II). For the first time, high concentration of Cu(II) is found to encourage the expression of PAH-RHDɑ and C23O genes during PHE degradation. Applying Sphingomonas PHE-1 in PHE-contaminated soils for bioaugmentation, the abundance of xylE gene was increased by the planting of ryegrass and the presence of Cu(II), which, in turn, benefited ryegrass growth. The best performance of PHE degradation and the highest abundance of xylE genes occurred in PHE-copper co-contaminated soils planted with ryegrass.

Item Type:
Journal Article
Journal or Publication Title:
Environmental Pollution
Additional Information:
This is the author’s version of a work that was accepted for publication in Environmental Pollution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental Pollution, 220, Part B, 2017 DOI: 10.1016/j.envpol.2016.11.037
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3000/3005
Subjects:
ID Code:
83137
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Deposited On:
25 Nov 2016 11:48
Refereed?:
Yes
Published?:
Published
Last Modified:
27 Sep 2020 03:36