Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor

Varzakakou, Maria and Roukas, Triantafyllos and Papaioannou, Emmanouil and Kotzekidou, Parthena and Liakopoulou-Kyriakides, Maria (2011) Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor. Preparative Biochemistry and Biotechnology, 41 (1). pp. 7-21. ISSN 1082-6068

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Abstract

The phenomenon of autolysis in Blakeslea trispora during carotene production from deproteinized hydrolyzed whey in an airlift reactor was investigated. The process of cellular autolysis was studied by measuring the changes in carotene concentration, dry biomass, residual sugars, pH, intracellular protein, specific activity of the hydrolytic enzymes (proteases, chitinase), and micromorphology of the fungus using a computerized image analysis system. All these parameters were useful indicators of autolysis, but image analysis was found to be the most useful indicator of the onset and progress of autolysis in the culture. Autolysis of B. trispora began early in the growth phase, continued during the stationary phase, and increased significantly in the decline phase. The morphological differentiation of the fungus was a result of the degradation of the cell membrane by hydrolytic enzymes. The biosynthesis of carotenes was carried out in the exponential phase, where the phenomenon of autolysis was not intense.

Item Type:
Journal Article
Journal or Publication Title:
Preparative Biochemistry and Biotechnology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1303
Subjects:
?? airlift reactorautolysisblakeslea trisporacarotenesdeproteinized hydrolyzed wheymorphologybiochemistrybiotechnology ??
ID Code:
82191
Deposited By:
Deposited On:
13 Oct 2016 12:36
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 16:26