Molecularly imprinted polymers for RGD selective recognition and separation

Papaioannou, Emmanouil and Koutsas, Christos and Liakopoulou-Kyriakides, Maria (2009) Molecularly imprinted polymers for RGD selective recognition and separation. Amino Acids, 36 (3). pp. 563-569. ISSN 0939-4451

Full text not available from this repository.

Abstract

Molecularly imprinted polymers that could recognize the tripeptide Arg-Gly-Asp have been produced with the use of two functional monomers and three different cross-linkers, respectively. Methacrylic acid and acrylamide were used as functional monomers and the role of the ethylene glycol dimethacrylate, trimethylpropane trimethacrylate and N,N?-methylene-bisacrylamide as crosslinking monomers, was investigated on their recognition capability. The % net rebinding and the imprinting factor values were obtained, giving for the methacrylic acid-trimethylpropane trimethacrylate polymer the highest values 12.3% and 2.44, respectively. In addition, this polymer presented lower dissociation constant (K D) value and the higher B max% of theoretical total binding sites than all the other polymers. Rebinding experiments with Lys-Gly-Asp, an analogue of Arg-Gly-Asp, and other different peptides, such as cholecystokinin C-terminal tri- and pentapeptide and gramicidin, further indicated the selectivity of methacrylic acid-trimethylpropane trimethacrylate copolymer for Arg-Gly-Asp giving specific selectivity factor values 1.27, 1.98, 1.31 and 1.67, respectively.

Item Type:
Journal Article
Journal or Publication Title:
Amino Acids
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1600/1605
Subjects:
ID Code:
82189
Deposited By:
Deposited On:
13 Oct 2016 12:30
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 09:59