Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora

Papaioannou, Emmanouil H. and Liakopoulou-Kyriakides, Maria and Christofilos, Dimitrios and Arvanitidis, Ioannis and Kourouklis, Gerasimos (2009) Raman spectroscopy for intracellular monitoring of carotenoid in blakeslea trispora. Applied Biochemistry and Biotechnology, 159 (2). pp. 478-487. ISSN 0273-2289

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Abstract

In the present study, we explore the feasibility of Raman spectroscopy for intracellular monitoring of carotenoid in filamentous fungi Blakeslea trispora. Although carotenoid production from this fungus has been extensively studied through various chromatographic methods and ultraviolet-visible spectroscopy, no intracellular monitoring has been demonstrated until now. The intensity of the Raman spectrum, and more conveniently that of the strongest ν 1 carotenoid band at ∼1,519 cm-1, exhibits a good linear correlation with the carotenoid content of the sample as determined by high-performance liquid chromatography (HPLC) and ultraviolet-visible (UV-Vis) spectroscopy. Our results suggest that Raman spectroscopy can serve as an alternative method for the study and quantification of carotenoid in batch-mated submerged cultivations of B. trispora and similar organisms. Although not as accurate as HPLC, it allows a rapid sampling and analysis, avoiding the prolonged and tedious classical isolation procedures required for carotenoid determination by HPLC and UV-Vis spectroscopy.

Item Type:
Journal Article
Journal or Publication Title:
Applied Biochemistry and Biotechnology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2300/2305
Subjects:
ID Code:
82180
Deposited By:
Deposited On:
13 Oct 2016 13:16
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 09:59