Production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles

Greiner, Ralf and Konietzny, Ursula and Blackburn, Daniel Menezes and Jorquera, Milko A. (2013) Production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles. Bioresource Technology, 142. pp. 375-383. ISSN 0960-8524

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Abstract

Phytases of different origin were covalently bound onto Fe3O4 magnetic nanoparticles (12 nm). Binding efficiencies of all three phytases were well above 70% relative to the number of aldehyde groups available on the surface of the magnetic nanoparticles. Temperature stability for all three phytases was enhanced as a consequence of immobilisation, whereas pH dependence of enzyme activity was not affected. Maximum catalytic activity of the immobilised phytases was found at 60°C (rye), 65°C (Aspergillus niger) and 70°C (Escherichia albertii). The immobilised enzymes exhibited the same excellent substrate specificities and unique myo-inositol phosphate phosphatase activities as their soluble counterparts. However, the catalytic turnover number dropped drastically for the immobilised phytases. The amount of the desired partially phosphorylated myo-inositol phosphate isomer could be easily controlled by the contact time of substrate solution and immobilised enzymes. The immobilised phytases showed a high operational stability by retaining almost full activity even after fifty uses.

Item Type:
Journal Article
Journal or Publication Title:
Bioresource Technology
Additional Information:
Copyright © 2013 Elsevier Ltd. All rights reserved.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2300/2311
Subjects:
ID Code:
81066
Deposited By:
Deposited On:
31 Aug 2016 15:08
Refereed?:
Yes
Published?:
Published
Last Modified:
17 Jun 2020 04:11