Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils

Jorquera, Milko A. and Crowley, David E. and Marschner, Petra and Greiner, Ralf and Fernández, María Teresa and Romero, Daniela and Menezes-Blackburn, Daniel and De La Luz Mora, María (2011) Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils. FEMS Microbiology Ecology, 75 (1). pp. 163-172. ISSN 0168-6496

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Abstract

Phytate is one of the most abundant sources of organic phosphorus (P) in soils, but must be mineralized by phytase-producing bacteria to release P for plant uptake. Microbial inoculants based on Bacillus spp. have been developed commercially, but few studies have evaluated the ecology of these bacteria in the rhizosphere or the types of enzymes that they produce. Here, we studied the diversity of aerobic endospore-forming bacteria (EFB) with the ability to mineralize phytate in the rhizosphere of pasture plants grown in volcanic soils of southern Chile. PCR methods were used to detect candidate phytase-encoding genes and to identify EFB bacteria that carry these genes. This study revealed that the phytate-degrading EFB populations of pasture plants included species of Paenibacillus and Bacillus, which carried genes encoding β-propeller phytase (BPP). Assays of enzymatic activity confirmed the ability of these rhizosphere isolates to degrade phytate. The phytase-encoding genes described here may prove valuable as molecular markers to evaluate the role of EFB in organic P mobilization in the rhizosphere.

Item Type: Journal Article
Journal or Publication Title: FEMS Microbiology Ecology
Additional Information: © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2400/2404
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 81065
Deposited By: ep_importer_pure
Deposited On: 31 Aug 2016 15:12
Refereed?: Yes
Published?: Published
Last Modified: 11 Feb 2020 09:01
URI: https://eprints.lancs.ac.uk/id/eprint/81065

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