Di(2-ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC-2spd cells

Fu, Guoqing and Dai, Juan and Zhang, Dayi and Zhu, Lishan and Tang, Xiao and Zhang, Ling and Zhou, Ting and Duan, Peng and Quan, Chao and Zhang, Zhibing and Song, Shizhen and Shi, Yuqin (2017) Di(2-ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC-2spd cells. Environmental Toxicology, 32 (3). pp. 1055-1064. ISSN 1520-4081

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Di(2-ethylhexyl) phthalate (DEHP), a plasticizer of synthetic polymers, is a well-known endocrine disrupting chemical (EDC) and reproductive toxicant. Addressing the unclear mechanism of DEHP-induced reproductive dysfunction, this study used GC-2spd cells to investigate the molecular mechanism involved in the DEHP-induced toxicity in the male reproductive system. The results indicated that the apoptotic cell death was significantly induced by DEHP exposure over 100 μM. Furthermore, DEHP treatment could induce oxidative stress in GC-2spd cells involving in the decrease of superoxide dismutase (SOD) activity (200 μM) and glutathione peroxidase (GSH-Px) activity (50 and 100 μM). In addition, DEHP induction also caused the elevated ratios of Bax/Bcl-2, release of cytochrome c and decomposition of procaspase-3 and procaspase-9 in GC-2spd cells. Taken together, our work provided the evidence that DEHP exposure might induce apoptosis of GC-2spd cells via mitochondria pathway mediated by oxidative stress. © 2016 Wiley Periodicals, Inc. Environ Toxicol, 2016.

Item Type:
Journal Article
Journal or Publication Title:
Environmental Toxicology
Additional Information:
This is the peer reviewed version of the following article: Fu, G., Dai, J., Zhang, D., Zhu, L., Tang, X., Zhang, L., Zhou, T., Duan, P., Quan, C., Zhang, Z., Song, S. and Shi, Y. (2017), Di(2-ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC-2spd cells. Environ. Toxicol., 32: 1055–1064. doi:10.1002/tox.22304 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/tox.22304/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords:
?? di(2-ethylhexyl) phthalate (dehp)gc-2spd cellsoxidative stressapoptosismitochondrial pathwayhealth, toxicology and mutagenesistoxicologymanagement, monitoring, policy and law ??
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16 Aug 2016 12:58
Last Modified:
15 Jul 2024 16:16